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Hafidz RN, Yaakob CM, Amin I, Noorfaizan A. Porous chitosan gelatin scaffold containing plasmid DNA encoding transforming growth factor-О±1 for chrondrocytes proliferation. Guo T, Zhao J, Chang J, Ding Z, Hong H, Chen J, Zhang J. Gelatine-Based Biomaterials and Their Biocompatibility: Review and Perspectives, Biomaterials Applications for Nanomedicine, Prof. International Food Research Journal 19 (3): 1175-1180. Differentiation of bovine and porcine gelatins in processed products via Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) and principal component analysis (PCA) techniques. Kinetics and mechanism of pepsinogen activation. Based on the results,each of the samples were tested contain of bovine gelatin respectively.ДЄl-Janabi J, JA Hartsuck, et al. Similar results were obtained on a sample of hard capsules with bands of protein fragments that were identical to bovine gelatinstandard. The results showed there were of specific bands of bovine gelatin with a molecular weight of 11,4 kDa 34 kDa 47kDa and specific bands of pork gelatin with a molecular weight of 24.7 kDa 28 kDa and 60 kDa. Hydrolysis time optimization throught applied to identify the source of hard gelatin capsules in the samples obtained from market and compared with the simulation of hard gelatin capsules.

Identification of gelatin hydrolyzate fragments were carried by molecular weight.

Gelatin hydrolyzateswere analyzed by SDS-PAGE to determine the optimal hydrolysis time. In the early stages, optimization of standards bovine and pork gelatin were hydrolyzed by pepsin at pH 4.5 and 60вЊ for 1 hour, 2 hours, and 3 hours. The main of study is determine the source of gelatin used in hard capsules by using SDS-PAGE ( Sodium Dodecyl Sulphate Gel electrophoresis Poly Acrylamide) method.

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One of gelatin source is came from collagen of the skin and bones of bovine or pork. Most of gelatin production remains largely derived from non-halal materials. Gelatin as the main ingredient of capsules is still a problem for a moslem.

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